Assay-kits

SensoLyte® 520 Renin Assay Kit Fluorimetric - 1 kit

$611.00
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  • Cat.Number : AS-72040
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Renin, a highly specific aspartyl protease, cleaves angiotensinogen, to yield angiotensin I, which is further converted into angiotensin II by ACE (Angiotensin Converting Enzyme). Angiotensin II constricts blood vessels leading to increased blood pressure. It also increases the secretion of ADH and aldosterone, and stimulates the hypothalamus to activate the thirst reflex. Since an overactive renin-angiotensin system leads to hypertension, renin is an attractive target for the treatment of this disease.
The SensoLyte® 520 Renin Assay Kit provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of renin activity using a 5-FAM/QXL™520 fluorescence resonance energy transfer (FRET) peptide. In the FRET peptide the fluorescence of 5-FAM is quenched by QXL™520. Upon cleavage into two separate fragments by renin, the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission = 490/520 nm. This assay is about fifty fold more sensitive than an EDANS/DABCYL-based assay and can detect 0.8 ng/ml renin.

Specifications

Packaging
Kits components
  • Component A: Renin substrate 5-FAM/QXL™520 FRET peptide Ex/Em=490 nm/520 nm upon cleavage: 1.5 mM DMSO solution, 50 µL Component B: 5-FAM, fluorescence reference standard: 1mM, 20 µL Component C: Human recombinant renin: 50 µL Component D: Assay buffer: 25 mL Component E: Renin Inhibitor Ac-HPFV- (Sta)-LF-NH2: 1mM DMSO solution, 4 µL
Chemistry
UniProt number
  • P00797
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components at -20°C expect for Component C, which should be stored at -80°C. Protect Components A and B from light. If used frequently, Component D can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 0.8 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use

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