Assay-kits

SensoLyte® 520 HCV Protease Assay Kit Fluorimetric - 1 kit

$480.00
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  • Cat.Number : AS-71145
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The SensoLyte® 520 HCV Assay Kit uses a new FRET peptide substrate that incorporates 5-FAM (fluorophore) and QXL™ 520 (quencher) for a continuous measurement of enzyme activities. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL™ 520. Upon cleavage of the FRET peptide by HCV NS3/4a protease, the fluorescence of 5-FAM is recovered and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, this 5-FAM/QXL™ 520-based FRET peptide shows less interference from autofluorescence of test compounds and cellular components. This assay is ten times more sensitive than an EDANS/DABCYL based assay and can detect 0.1 pmole of HCV NS3/4a protease. The assays are performed in a convenient 96-well or 384-well microplate format.

Specifications

Packaging
Kits components
  • Component A: HCV NS3/4A protease substrate Ex/Em=490 nm/520 nm upon cleavage: 120 µL Component B: 5-FAM, fluorescence reference standard Ex/Em=490 nm/520 nm upon cleavage: 100 µM, 5 µL Component C: 2X Assay buffe:r 10 mL Component D: Stop solution: 10 mL Component E DTT: 1 M, 0.5 mL Component F: Pep4AK: 50 µL, 600 µM
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components at -20°C. Protect Components A and B from light. Components C and D can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 0.1 pmole
Research Area
Sub-category Research Area
Usage
  • Research use

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